Table 1. Comparison of the efficiency of different BAL protocols.

Total cell number in BAL fluid obtained in cited studies. Only counts from WT animals (or comparable conditions, such as control-treated WT animals) were considered. WT denotes wild-type mouse.

Mouse strain	Total cell number	Volume	Buffer conditions	References
WT (4 weeks)	20,000	2 x 0.4 ml	0.5 mM EDTA	Yu et al., 2017
WT (control)	40,000	2 x 0.8 ml		Yuan et al., 2015
WT (floxed)	30-40,000	3 x 0.4 ml		Schneider et al., 2014b
WT (control)	50,000	2 x 1 ml	EDTA, 4 °C	Machiels et al., 2017
WT	60,000	3 x 0.4 ml		Schneider et al., 2014a
WT (6 weeks)	50-150,000	3 x 1 ml	0.5 mM EDTA	van de Laar et al., 2016
WT (1 year)	100-200,000	3 x 1 ml	0.5 mM EDTA	van de Laar et al., 2016
WT (control)	250,000	5 x 1 ml	5 mM EDTA	Qian et al., 2018
WT (1 year)	250-300,000	5 x 1 ml		Suzuki et al., 2014
WT	300-500,000	3 x 2 ml	0.5 mM EDTA, 37 °C	Zhang et al., 2008
WT	400-500,000	3 x 1 ml	2% BSA, 2 mM EDTA	Dong et al., 2018
WT (6-8 weeks)	500-700,000	10 x 1 ml	0.5% FBS, 2 mM EDTA, 37 °C	Soucie et al., 2016, Imperatore et al., 2017 and this study