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. Author manuscript; available in PMC: 2019 Sep 11.
Published in final edited form as: EMBO Rep. 2019 Mar 4;20(4):e46620. doi: 10.15252/embr.201846620

Figure 3. miR-132 and its targets p300 and BTAF1 control RP expression.

Figure 3

A. mRNA levels of indicated RP transcripts determined by qRTPCR in MEFs transfected with Non-targeting control (NTC) mimics (white) or miR-132-3p mimics (grey).

B. p300 and BTAF1 protein levels in MEF transfected with NTC mimics or miR-132-3p mimics determined by Western blot. GAPDH was used as a loading control. Right panel indicates mean + SEM of 4 experiments.

C. mRNA levels of indicated RP transcripts determined by qRTPCR in MEFs transfected with NTC siRNA (white) or p300 siRNA (grey).

D. mRNA levels of indicated RP transcripts determined by qRTPCR in MEFs transfected with NTC siRNA (white) or BTAF1 siRNA (grey).

E. mRNA levels of indicated RP transcripts determined by qRTPCR in MEFs transfected with NTC or miR-132-3p mimics and NTC siRNA or p300 or BTAF1 siRNAs for 48h. Levels are normalised to cells transfected with NTC siRNA and NTC mimic.

F. Puromycin incorporation (following 10-minute pulse and 50-minute chase) determined by western blot in MEFs transfected with NTC or miR-132-3p or miR-212-3p mimics.

Data information: Statistical significance is determined by unpaired t-test from 4-6 experiments. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.