A. mRNA levels of indicated RP transcripts determined by qRTPCR in MEFs transfected with NTC mimics (white) or miR-212-3p mimics (grey). Cultures performed in triplicate. Statistical significance determined by t-test.
B. Protein levels determined by western blot of RPL27 and RPS10 in MEFs transfected with NTC, miR-132-3p, or miR-212-3p mimics for 48h.
C. Top enriched molecular function GO terms for miR-132/212-3p predicted target genes. Predictions retrieved from Targetscan, total context score <-0.1.
D. Protein levels determined by western blot of BTAF1 and p300 in EL4 cells transfected with NTC, miR-132-3p, or miR-212-3p mimics for 48h.
Data information: * p<0.05, ** p<0.01.