Expanded View Figure EV4. miR-132 controls the balance between IL-10 and IFN_γ production in CD4⁺ T cells.

A. Intracellular cytokine staining of WT or miR-132^-/- splenic live CD45.2⁺ TCRβ⁺ CD4⁺ cells for IFNγ and IL-10 from d0 naïve and day 28 L. donovani infected mice following ex vivo stimulation (4hrs) with PMA and ionomycin.

B. Antigen-specific IFNγ and IL-10 production by splenic CD4⁺ T cells from CD45.2⁺ L. donovani-infected WT (blue) or miR-132^-/- (red) mice was assessed as described in materials and methods. Cells were cultured for 3 days in the absence of exogenous stimulation (“Neg”, open circles) or with parasite antigen (“Ag”, closed circles), after which cytokine production by CD4⁺ T cells from infected (“CD45.2”) or naïve mice (“CD45.1”) was determined. Representative FACS plots for antigen-stimulated CD45.2⁺ cells are shown. Significance determined by unpaired t-test, purified CD4⁺ T cells from 4-5 mice per group.