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. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: J Immunol. 2019 Oct 4;203(10):2724–2734. doi: 10.4049/jimmunol.1900640

Figure 4. NLRP3 regulates Ym1 following infection with N. brasiliensis.

Figure 4

After infection of WT and Nlrp3−/− mice with N. brasiliensis (Nb), (A) IL-4 protein levels were measured in lung tissues by ELISA on day 2 post-infection (normalised to tissue weight). (B) Chil3 (Ym1) gene expression was measured by qRT-PCR on day 7 post-infection (log2 expression relative to Rpl13a). (C) BALF Ym1 was measured by ELISA and (D) intracellular expression by geometric mean fluorescence intensity (gMFI) (fold change over WT control) by alveolar (alv.) Mφs and neutrophils were quantified by flow cytometry on day 7 post-infection. Data are representative (A; mean ± s.e.m.) or pooled (B–D; mean ± s.e.m.) from 3 individual experiments with 3-5 mice per group (per experiment). *P<0.05, **P<0.01, (one-way ANOVA and Tukey-Kramer post hoc test).