Figure 6. NLRP3 regulates resident Mφ alternative activation by IL-4.

WT and Nlrp3^−/− mice were injected with either PBS or IL-4 complex (IL-4c) and (A) peritoneal CD11b⁺ cells containing F4/80^lo and F4/80^hiTim4⁺ Mφs where analysed by flow cytometry after 24 hours. Frequency and geometric mean fluorescence intensity (gMFI) of intracellular Ym1 and RELM-α were determined in (B) resident F4/80^hiTim4⁺ Mφs and (C) monocyte-derived F4/80^lo Mφs. Data are representative (B left panel; mean ± s.e.m.) or pooled (B right panel and C; mean ± s.e.m.) from 3 individual experiments with 3-5 mice per group (per experiment). *P<0.05, ****P<0.0001 (one-way ANOVA and Tukey-Kramer post hoc test).