| qYD21 |
GCCACTTCGCCGTTGATGATG |
qPCR Forward |
crh-1a |
| qYD20 |
TCCTCCGGCTCCTTCTTCATC |
qPCR Reverse |
crh-1a |
| qYD179 |
GTAGATGCTTCACCATTACAGTTT |
qPCR Forward |
crh-1b |
| qYD23 |
GGCAAGACGAGTGGCTGATT |
qPCR Reverse |
crh-1b |
| qYD22 |
ATGTCAGCGAAAGGTAACGGATC |
qPCR Forward |
crh-1c |
| qYD20 |
TCCTCCGGCTCCTTCTTCATC |
qPCR Reverse |
crh-1c |
| qYD24 |
GTACCCAACAGCAACACGG |
qPCR Forward |
crh-1d |
| qYD25 |
CCGCCGTTTCTAGATCTTTGAG |
qPCR Reverse |
crh-1d |
| qYD19 |
ATGATGTTCCTCAGGGCATTACAA |
qPCR Forward |
crh-1e |
| qYD20 |
TCCTCCGGCTCCTTCTTCATC |
qPCR Reverse |
crh-1e |
| qYD26 |
ATGGAGTCACTGGTTTTCAATGG |
qPCR Forward |
crh-1f |
| qYD27 |
GTACGGATTGTTGTTGGGATGG |
qPCR Reverse |
crh-1f |
| qYD09 |
TACTCTTTCACCACCACCGC |
qPCR Forward |
act-1 |
| qYD10 |
ACGGTGATGACTTGTCCGTC |
qPCR Reverse |
act-1 |
| YD157 |
TGGAAGGAGGAGGAGATGGAAA |
Genotyping Forward (external) |
crh-1(tz2) |
| YD158 |
GCAGTACAGCTCTTTCAGCGTT |
Genotyping Forward (internal) |
crh-1(tz2) |
| YD159 |
AATTCGGCACAACGGACTGG |
Genotyping Reverse (external) |
crh-1(tz2) |
| YD172 |
ATTACCTGCAGGCTGCAGGTCGACTCTAGAG |
Cloning Forward |
phsp16.41
|
| YD173 |
GGCAGCTAGCATTTTTTCTACCGGTACCAATAC |
Cloning Reverse |
phsp16.41
|
| YD165 |
ATTAGCTAGCATGGCCACAATGGCGAGCAC |
Cloning Forward |
crh-1a |
| YD164 |
ATTACCATGGTCACATTCCGTCCTTTTCCTTTC |
Cloning Reverse |
crh-1a |
| YD165 |
ATTAGCTAGCATGGCCACAATGGCGAGCAC |
Cloning Forward |
crh-1b |
| YD164 |
ATTACCATGGTCACATTCCGTCCTTTTCCTTTC |
Cloning Reverse |
crh-1b |
| YD166 |
ATTAGCTAGCATGTCAGCGAAAGGTAACGGAT |
Cloning Forward |
crh-1c |
| YD164 |
ATTACCATGGTCACATTCCGTCCTTTTCCTTTC |
Cloning Reverse |
crh-1c |
| YD167 |
ATTAGCTAGCATGGCCACAATGGCGAGCAC |
Cloning Forward |
crh-1d |
| YD164 |
ATTACCATGGTCACATTCCGTCCTTTTCCTTTC |
Cloning Reverse |
crh-1d |
| YD168 |
ATTAGCTAGCATGATGTTCCTCAGGGCATTACA |
Cloning Forward |
crh-1e |
| YD164 |
ATTACCATGGTCACATTCCGTCCTTTTCCTTTC |
Cloning Reverse |
crh-1e |
| YD169 |
ATTAGCTAGCATGGAGTCACTGGTTTTCAATGG |
Cloning Forward |
crh-1f |
| YD164 |
ATTACCATGGTCACATTCCGTCCTTTTCCTTTC |
Cloning Reverse |
crh-1f |
| YD180 |
CATAATAACCGGTAAAATGTACCCATACGACGTTCCAG |
Cloning Forward |
3xHA |
| YD181 |
CATAGCTAGCAGCGTAATCTGGGACGTCA |
Cloning Reverse |
3xHA |
| YD320 |
ATCAGCATGCATCTTCAGATGGGAGCAGTGG |
Cloning Forward |
prab-3
|
| YD321 |
GCATGGATCCAAACTTGTCATCTGAAAATAGGGCT |
Cloning Reverse |
prab-3
|
| YD260 |
ATAGCCTGCAGGAAGTGGACACTGAGAGAGAGAG |
Cloning Forward |
pnmr-1
|
| YD261 |
ATAGCCCGGGCTGTAACAAAACTAAAGTTTGTCGTGTTC |
Cloning Reverse |
pnmr-1
|
| AB139 |
GCGTCGACAAGTGACACCACGCTCACA |
Cloning Forward |
prig-3
|
| AB140 |
CCCCCCGGGAGCTGTGAAATTTTTAGGCAGT |
Cloning Reverse |
prig-3
|
| YD313 |
ACGTGCATGCGTTAAATATGTGGCCACAATGACAATTAT |
Cloning Forward |
pgcy-13
|
| YD314 |
ATCAGGATCCGTCCTGAAAAATTATTGAAAGTTTGTAATAAG |
Cloning Reverse |
pgcy-13
|
| YD253 |
ATACCTGCAGGGAATTTAATAACTAATACATATTATTTGGCACACTC |
Cloning Forward |
pcrh-1e
|
| YD254 |
ATAGGATCCATTATTCAGAAATTGAATAGAGAATTAGAATTAGAAAC |
Cloning Reverse |
pcrh-1e
|
| YD318 |
CTGCAAGCTTCACTTTTCTACGTTTCCATAATAATTACATAG |
Cloning Forward |
pcrh-1a
|
| YD319 |
ATACGGATCCAGCCGTGAGATGTCCGC |
Cloning Reverse |
pcrh-1a
|
| YD205 |
AGTAAGCTTGTTTACGCTCGCGAGGAGAC |
Cloning Forward |
pstr-2
|
| YD206 |
AGTCTGCAGTTTTATGGATCACGAGTATTCG |
Cloning Reverse |
pstr-2
|
| crYD35 |
ATCAGGTCTCCTCTTCTTTTCTAATACTTACCCTGGTTTTAGAGCTAGAAATAGCAAG |
Guide RNA |
crh-1e deletion |
| crYD65 |
ATCAGGTCTCCTCTTCAAAATGTCAGCGAAAGGTAAGTTTTAGAGCTAGAAATAGCAAG |
Guide RNA |
crh-1c deletion |
| crYD48 |
ATCAGGTCTCCTCTTGAGCATCATCAACGGCGAAGGTTTTAGAGCTAGAAATAGCAAG |
Guide RNA |
crh-1a,b,d deletion |
| crYD106 |
ATCAGGTCTCCTCTTTGGGCTCGAGCTGTAGCCGCGTTTTAGAGCTAGAAATAGCAAG |
Guide RNA |
crh1(K247R/K266R) |
| crYD01 |
GTTTCTCGAGCCATGGTTACCGGTCTC |
Guide RNA |
Universal |
| crYD44 |
ATACGCATGCACACCTAAAATTTTCAGATTGAGTCTGG |
Forward |
5′ Homology arm crh-1e deletion |
| crYD45 |
ATCAACTAGTTGCGCCCTGTTGAACTGGGA |
Reverse |
5′ Homology arm crh-1e deletion |
| crYD46 |
ATCAGGGCCCAGAAAAGATTAGTGTGTGTGTGTTTG |
Forward |
3′ Homology arm crh-1e deletion |
| crYD47 |
ATCACTTAAGCGAGTTGGTTTTATTACACAGCAGAG |
Reverse |
3′ Homology arm crh-1e deletion |
| crYD59 |
ATCAACATGTGCGAATCGCTGATTGGTTGCAATTC |
Forward |
5′ Homology arm crh-1c deletion |
| crYD60 |
ATCAACTAGTTTTATACTCGTTTTGGTGTGCAAAAAGGC |
Reverse |
5′ Homology arm crh-1c deletion |
| crYD61 |
ATCAGGGCCCAATGACACAAGAATGCGAAAACATCT |
Forward |
3′ Homology arm crh-1c deletion |
| crYD62 |
ATCACTTAAGTTCGGCCAATTTTGCGAATTTTAAG |
Reverse |
3′ Homology arm crh-1c deletion |
| crYD50 |
ATCAGCATGCTCTCTTTCACCGCCAATTTTTGTG |
Forward |
5′ Homology arm crh- 1a,b,d deletion |
| crYD51 |
ATCAACTAGTGAGATGTCCGCCACTTATTTTTGTTTA |
Reverse |
5′ Homology arm crh- 1a,b,d deletion |
| crYD52 |
ATCAGGGCCCAGTAGAGTTTTAGTGGAAAAATTTCGAG |
Forward |
3′ Homology arm crh- 1a,b,d deletion |
| crYD53 |
ATCACTTAAGCGATATGAGGGCCTCCTATTAAGTT |
Reverse |
3′ Homology arm crh- 1a,b,d deletion |
| crYD74 |
ACTCGCATGCGAAGTGCAATGAAGCCAATGTTGG |
Forward |
5′ Homology arm crh- 1(K247R/K2 66R) |
| crYD90 |
ACTAACTAGTCAAATTTCCGCTCCAAA |
Reverse |
5′ Homology |
|
AAATTACCTTACGTTTTCTGCGGCACTCTTTCGCTGCCTCTCGATT |
|
arm crh- 1(K247R/K2 66R) |
| crYD91 |
ACTCGGGCCCGAGGGAAAAAAATTATTTTTTGGCTGAAAAATTGA |
Forward |
3′ Homology arm crh- 1(K247R/K2 66R) |
| crYD92 |
ACGACTTAAGTGAGTGCTTTGTTCTGATTTTCCAGC |
Reverse |
3′ Homology arm crh- 1(K247R/K2 66R) |
