| Antibodies |
| CD4 (RM4-5) |
BD Biosciences |
Cat#560782; RRID:AB_1937315 |
| PD-1 (J43) |
Thermo Fisher |
Cat#11-9985-82; RRID:AB_465472 |
| Ki67 (SolA15) |
Thermo Fisher |
Cat#12-5698-80; RRID:AB_11149672 |
| CD45 (30-F11) |
BD Biosciences |
Cat#561487; RRID:AB_561487 |
| CD45.1 (A20) |
Biolegend |
Cat#110716; RRID:AB_10660302 |
| CD45.1 (A20) |
Thermo Fisher |
Cat#25-0453-81; RRID:AB_469628 |
| CD45.2 (clone 104) |
BD Biosciences |
Cat#562130; RRID:AB_10896649 |
| FoxP3 (FJK-15 s) |
Thermo Fisher |
Cat#11-5773-82; RRID:AB_465243 |
| CXCR5 (2G8) |
BD Biosciences |
Cat#551960; RRID:AB_394301 |
| CD19 (ID3) |
Thermo Fisher |
Cat#25-0193-82; RRID:AB_657663 |
| CD19 (ID3) |
BD Biosciences |
Cat#561113; RRID:AB_10563071 |
| B220 (RA3-6B2) |
Thermo Fisher |
Cat#12-0452-82; RRID:AB_465671 |
| B220 (RA3-6B2) |
Biolegend |
Cat#103236; RRID:AB_893354 |
| Fas (Jo2) |
BD Biosciences |
Cat#563647; RRID:AB_2738346 |
| GL7 (GL7) |
BD Biosciences |
Cat#553666; RRID:AB_394981 |
| CD86 (GL1) |
Biolegend |
Cat#105014; RRID:AB_439783 |
| CXCR4 (L276F12) |
Biolegend |
Cat#146509; RRID:AB_2562786 |
| CD138 (281-2) |
Biolegend |
Cat#142503; RRID:AB_10915989 |
| CD138 (281-2) |
BD Biosciences |
Cat#562610; RRID:AB_11153126 |
| IgD (11-26c.2a) |
Biolegend |
Cat#405711; RRID:AB_1937245 |
| IgG1 (A85-1) |
BD Biosciences |
Cat#550331; RRID:AB_2296342 |
| IgM (II/41) |
Thermo Fisher |
Cat#25-5790; RRID:AB_469655 |
| PD-L2/CD273 (122) |
Thermo Fisher |
Cat#11-9972-82; RRID:AB_465462 |
| CD80 (16-10A1) |
Biolegend |
Cat#104713; RRID:AB_1240733 |
| Tbet (4B10) |
Thermo Fisher |
Cat#50-5825-80; RRID:AB_10597459 |
| GATA3 (16E10A23) |
Biolegend |
Cat#653809; RRID:AB_2563216 |
| BCL6 (K112-91) |
BD Biosciences |
Cat#562198; RRID:AB_11153136 |
| IL-17a (TC11-18H10) |
BD Biosciences |
Cat#560220; RRID:AB_1645194 |
| CD38 (90) |
Biolegend |
Cat#102727 RRID:AB_2616967 |
| Gr-1 (RB6-8C5) |
Biolegend |
Cat#108437 RRID:AB_2562214 |
| F4/80 (BM8) |
Biolegend |
Cat#123135 RRID:AB_2562622 |
| IL-21R (2A9) |
Bioxcell |
Cat#BE0258; RRID:AB_2687737 |
| Chemicals, Peptides, and Recombinant Proteins |
| Ovalbumin (OVA) |
Hyglos |
Cat#321001 |
| NP-chicken gamma globulin (NP-CGG) (Ratio 20) |
Biosearch Technologies |
Cat#N-5055C-1 |
| NP-Ovalbumin (NP-OVA) (Ratio 20 or 17) |
Biosearch Technologies |
Cat# N-5051-10-BS |
| Aluminum potassium sulfate dodecahydrate (Alum) |
Sigma |
Cat#31242-500G-D |
| Strepavidin APC |
BD Biosciences |
Cat#554067 |
| Strepavidin APC-Cy7 |
Biolegend |
Cat#405208 |
| NP-PE (Ratio 28) |
Biosearch Technologies |
Cat#N-5070-1 |
| 7-amino-actinomycin D (7-AAD) |
Biolegend |
Cat#420403 |
| AnnexinV-PE |
Biolegend |
Cat#640908 |
| Sodium Hydroxide |
Fisher |
Cat# S318-1 |
| Sheep Red Blood Cells (SRBCs) |
Labor Dr. Merck, Ochsenhausen |
Cat#E-400 |
| Critical Commercial Assays |
| Thermo Fisher FoxP3 Transcription Factor Staining Buffer Set |
Fisher |
Cat#00-5523-00 |
| RNA Isolation Kit |
QIAGEN |
Cat#GR8RNA |
| Omniscript RT Kit 200 |
QIAGEN |
Cat#205113 |
| qRT-PCR Kit (Rox) |
Bio & SELL |
Cat#BS76.520.5000 |
| Chromatrap® pro G ChIP spin column kit |
Direct 7Bioscience |
Cat#500190 |
| Bio-Plex Pro Mouse Cytokine IL-21 |
Biorad |
Cat#171GA003M |
| Experimental Models: Cell Lines |
| Jurkat T cells |
ATCC |
ATCC-TIB152 |
| Experimental Models: Organisms/Strains |
|
Nr2f6-deficient mice (C57BL/6) |
Hermann-Kleiter et al., 2008 |
N/A |
| Wild-type, C57BL/6N |
Charles River |
Strain Code#027 |
|
Nr2f6+/+ OT-II, C57BL/6-Tg(TcraTcrb) 425Cbn/Crl |
Charles River |
Strain Code#643 |
|
Nr2f6−/− OT-II |
In House |
N/A |
| B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) |
Jackson |
Stock# 002014; RRID:IMSR_JAX:002014 |
| B6.SJL x C57BL/6 (CD45.1/CD45.2) |
In House |
N/A |
| Oligonucleotides |
| See Table S1 for EMSA Oligonucleotide sequences |
This paper |
N/A |
| CNS +23 F-ACTTCCACTGGTCAGGGTCT |
Synthesized by Eurofins |
N/A |
| CNS +23 R-AGCAATGGTCAATTGTTGTGGG |
Synthesized by Eurofins |
N/A |
| CNS −36 F-CTCCAGTGACCCCATTCTCC |
Synthesized by Eurofins |
N/A |
| CNS −36 R-TTGCTCTCGCCAAGTGGAAA |
Synthesized by Eurofins |
N/A |
| Prom −1- −2.2kb F-CAGCAGCGTCCCTTACAGAA |
Synthesized by Eurofins |
N/A |
| Prom −1- −2.2kb R-CTTCTGCAGGAGATGAGCATT |
Synthesized by Eurofins |
N/A |
| qRT-PCR primers Il21
|
Applied Biosystem |
Cat#Mm00517640_m1 |
| qRT-PCR primers Il4
|
Applied Biosystem |
Cat#Mm00445259_m1 |
| qRT-PCR primers Il2
|
Applied Biosystem |
Cat#Mm00434256_m1 |
| Recombinant DNA |
| Nr2f6-pEFNeos construct |
Hermann-Kleiter et al., 2008 |
N/A |
| Software and Algorithms |
| FlowJo 10 |
TreeStar |
RRID:SCR_008520 |
| Fiji (ImageJ) |
N/A |
http://fiji.sc; RRID:SCR_002285 |
| Prism 7 |
Graphpad |
https://www.graphpad.com/; RRID:SCR_002798 |
| R |
N/A |
https://www.r-project.org/ |
