Evaluation of image quality using mini IQ NEMA NU-4 phantoms. Mini IQ phantoms were designed specifically for the evaluation of the four-bed mouse hotel (A). Dimensions are shown in mm. Mini IQ phantoms were filled with ~3.7MBq of 18F-FDG and imaged over 20 mins. Following reconstruction, NEMA NU-4 tests were performed. The RC values of 1-5 mm rods (B), SORs (C) and uniformity values (D) of phantoms imaged using the four-bed mouse hotel and a single mouse bed were acquired and compared. (E). Representative sagittal and axial PET images of 0-20 min summed activity of imaging configurations 1, 2 and 3, reconstructed using 10 iterations and 6 subsets. Red lines represent the tolerable limits set by NEMA NU-4. For (B), *** = P < 0.001 for configuration 1 vs configuration 2; ### = P < 0.001 for configuration 1 vs configuration 3; for (C), *** = P < 0.001 for configuration 1 SORwater vs configuration 2 and 3; ### = P < 0.001 for configuration 1 SORair vs configuration 2 and 3.