Figure 9.
C. glabrata secretome has no effect on the production of the proinflammatory cytokine IL-1β by THP-1 macrophages. PMA-differentiated THP-1 cells were either left untreated or incubated with wild-type and Cgyps1-11Δ secretome for 24 h. Levels of secreted IL-1β were measured in 100 μL of culture media using the BD OptEIA ELISA kit. Data (mean ± standard deviation (SD); n = 2) represent relative amounts of IL-1β produced by secretome-co-incubated THP-1 cells compared to the untreated THP-1 macrophages (considered as 1.0).