Fig 6.

rpoC^A494V is necessary and sufficient to cause a slowdown in growth and confers GASP in a background with attenuated σ^S activity. (A) Comparison of maximum growth rates of strains carrying rpoC^WT (marked +) and rpoC^A494V (marked *) in different backgrounds. Maximum growth rates were calculated from growth data as described in Materials and Methods. (B) Comparison of lag times of strains carrying rpoC^WT (+) and rpoC^A494V (*) in different backgrounds. Lag times were calculated from growth curve data as in Materials and Methods. (C) Competition dynamics between strains carrying rpoC^WT and rpoC^A494V in different backgrounds. Left panel shows scenarios where the rpoC^WT strain is inoculated in 1,000-times-higher frequency than the rpoC^A494V strain. Right panel shows competing strains grown individually in the same-age medium as controls. Data shown only for competitions performed in 10-day-old spent medium. Other medium conditions and starting frequencies are provided at https://doi.org/10.6084/m9.figshare.12001212.v1.