Table 1. Eluate characteristics for Plasmodium falciparum-spiked whole blood-derived samples.
Column | Parasitemia | Eluate volume (mean ± SEM, μl) | [DNA] (mean ± SEM, ng/μl) | Total DNA yield (mean ± SEM, ng) | n |
---|---|---|---|---|---|
Q100 | Overall | 197 ± 1.4 | 22.4 ± 1.6 | 4420 ± 340 | 12 |
1% | 22.1 ± 1.5 | 4320 ± 290 | 3 | ||
0.1% | 21.4 ± 2.2 | 4260 ± 500 | 3 | ||
0.01% | 24.5 ± 0.5 | 4880 ±170 | 3 | ||
0% | 21.5 ± 1.1 | 4210 ± 210 | 3 | ||
Q5 | Overall | 194 ± 0.4 | 1.06 ± 0.06 | 205 ± 11 | 12 |
1% | 1.06 ± 0.02 | 204 ± 4.5 | 3 | ||
0.1% | 1.09 ± 0.12 | 211 ± 23 | 3 | ||
0.01% | 1.08 ± 0.03 | 209 ± 5.6 | 3 | ||
0% | 1.02 ± 0.05 | 197 ± 10 | 3 | ||
DNA-XT | Overall | 42 ± 0.4 | 0.63 ± 0.04 | 26.3 ± 1.7 | 12 |
1% | 0.65 ± 0.03 | 28.4 ± 1.1 | 3 | ||
0.1% | 0.59 ± 0.05 | 24.8 ± 2.3 | 3 | ||
0.01% | 0.68 ± 0.05 | 28.9 ± 2.6 | 3 | ||
0% | 0.55 ± 0.07 | 22.8 ± 3.7 | 3 |