Table 2. Eluate characteristics for purified Leishmania donovani amastigote samples.
Column | Eluate volume (μl) | [DNA] (ng/μl) | Total DNA yield (ng) | |
---|---|---|---|---|
Q5 | Mean ± SEM | 193 ± 1.2 | 0.06 ± 0.002 | 11 ± 0.4 |
A | 191 | Too low | nd | |
B | 195 | 0.06 | 11 | |
C | 193 | 0.05 | 10 | |
DNA-XT | Mean ± SEM | 42 ± 0.3 | 0.17 ± 0.01 | 7.3 ± 0.2 |
A | 42 | 0.16 | 6.6 | |
B | 42 | 0.11 | 7.2 | |
C | 43 | 0.19 | 8.1 |
nd: Not determined.