REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Strep-tag II antibody	Abcam	Cat#ab76949; RRID:AB_1524455
Anti-Histone H3 antibody	Abcam	Cat#ab10799; RRID:AB_470239
Anti-GFP from mouse IgG1k (used for YFP detection)	Sigma	Cat#11814460001; RRID:AB_390913
GFP antibody rabbit polyclonal (used for YFP detection)	Chromotek	Cat#PABG1; RRID:AB_2749857
GAPDH (14C10) Rabbit mAb	Cell Signaling	Cat#2118; RRID:AB_561053
Anit-H1.10 antibody	Abcam	Cat#ab11079; RRID:AB_2295032
Anti-SPRTN mAB (6F2)	Stingele lab	Clone6F2
Goat Anti-Rat Immunoglobulins/HRP	Sigma	Cat#A9037; RRID:AB_258429
Goat Anti-Mouse Immunoglobulins/HRP	Dako	Cat#P0447; RRID:AB_2617137
Swine Anti-Rabbit Immunoglobulins/HRP	Dako	Cat#P0399; RRID:AB_2617141
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Scientific	Cat#A-11001; RRID:AB_2534069
Bacterial and Virus Strains
BL21(DE3)	Thermo Scientific	Cat#C600003
Chemicals, Peptides, and Recombinant Proteins
16% Formaldehyde (w/v), Methanol-free	Thermo Scientific	Cat#28906
InstantBlue	Sigma	Cat#ISB1L
Doxycycline Hyclate	Sigma	Cat#D9891
ProLong Gold Antifade Reagent	Thermo Fisher	Cat#P10144
DAPI Solution	Thermo Fisher	Cat#62248
4x NuPAGE LDS sample buffer	Thermo Scientific	Cat#NP0007
Phusion HF enzyme	NEB	Cat#M0530
UltraPure BSA	Thermo Scientific	Cat#AM2616
Histone H1° Human	NEB	Cat#M2501S
Protein G	BioVision	Cat#6510
Lipofectamine 2000	Thermo Scientific	Cat#11668030
IGEPAL	Sigma	Cat#I8896
Biotin	IBA Lifesciences	Cat#2-1016-005
Pefabloc SC	Merck	Cat#11585916001
TCEP	ROTH	Cat#HN95.2
cOmplete EDTA-free protease inhibitor cocktail	Merck	Cat#4693132001
Critical Commercial Assays
proFIRE Amine Coupling Kit	Dynamic Biosensors	Cat#PF-NH2-1
NucleoSpin® Gel and PCR Clean-up	MACHEREY-NAGEL	Cat#740609
Quant-iT PicoGreen dsDNA Assay Kit	Thermo Scientific	Cat#P11496
GeneJET Genomic DNA purification kit	Thermo Scientific	Cat#K0722
SsoAdvanced Universal SYBR Green Supermix	Bio-Rad	Cat#172527
Experimental Models: Cell Lines
Human HeLa Flp-In-T-REx	The Francis Crick Institute Cell Services	N/A
Human HAP1	Thijn Brummelkamp, NKI Amsterdam	N/A
Oligonucleotides
Oligonucleotide sequences used in this study are provided in Table S1	N/A	N/A
Recombinant DNA
Ds ΦX174 phage DNA RFI	NEB	Cat#N3021S
Ss ΦX174 phage DNA virion	NEB	Cat#N3023S
pMAX-GFP	LONZA	Cat#VDC-1040
pNIC-STREP-ZB-SPRTN-WT	This study	N/A
pNIC-STREP-ZB-SPRTNEquation (E112Q)	This study	N/A
pNIC-STREP-ZB-SPRTN-ZBD1* (Y179A_W197A)	This study	N/A
pNIC-STREP-ZB-SPRTN-ZBD2* (R185A)	This study	N/A
pNIC-STREP-ZB-SPRTN-BR* (K220A_K221E_G222A_K223A)	This study	N/A
pNIC-STREP-ZB-SPRTN-EQ-ZBD1* (E112Q_Y179A_W197A)	This study	N/A
pNIC-STREP-ZB-SPRTN-EQ-ZBD2* (E112Q_R185A)	This study	N/A
pNIC-STREP-ZB-SPRTN-EQ-BR* (E112Q_K220A_K221E_G222A_K223A)	This study	N/A
pNIC-STREP-ZB-SPRTN-EQ-ZBD2*-BR*(E112Q_R185A_K220A_K221E_G222A_K223A)	This study	N/A
pNIC-STREP-ZB-SPRTN-ZBD-BR(aa151-245)	This study	N/A
pNIC-STREP-ZB-SPRTN-ZBD (aa151-215)	This study	N/A
pcDNA5-FRT/TO-YFP-SPRTN-WT-Strep	Stingele et al., 2016	N/A
pcDNA5-FRT/TO-YFP-SPRTNEquation (E112Q)-Strep	Stingele et al., 2016	N/A
pcDNA5-FRT/TO-YFP-SPRTN-ZBD1* (Y179A_W197A)-Strep	This study	N/A
pcDNA5-FRT/TO-YFP-SPRTN-ZBD2*(R185A)-Strep	This study	N/A
pcDNA5-FRT/TO-YFP-SPRTN-BR* (K220A_K221E_G222A_K223A)-Strep	This study	N/A
pOG44	Thermo Scientific	Cat#V600520
pBABE-puro	Addgene	Cat#1764
pBABE-puro-SPRTN-WT-Strep	This study	N/A
pBABE-puro-SPRTNEquation (E112Q)-Strep	This study	N/A
pBABE-puro-SPRTN-ZBD1* (Y179A_W197A)-Strep	This study	N/A
pBABE-puro-SPRTN-ZBD2* (R185A)-Strep	This study	N/A
Software and Algorithms
Prism 7	GraphPad Software	https://www.graphpad.com/
ImageJ	NIH	https://imagej.net/Fiji/Downloads
Adobe Photoshop CC2018	Adobe	https://www.adobe.com/es/products/photoshop.html
Other
HiTrap Heparin HP affinity columns	GE Healthcare	Cat#17040701
PD-10 Desalting columns	GE Healthcare	Cat#17085101
Strep-Tactin®XT Superflow®high capacity cartridges	IBA Lifesciences	Cat#2-4026-001
HiLoad 16/600 Superdex 200 pg column	GE Healthcare	Cat#GE28-9893-35
10 kDa cutoff Amicon Ultra centrifugal filters	Merck	Cat#UFC801096
GFP-Trap Magnetic Agarose	Chromotek	Cat#gtma-10