Fig. 2.

Majority of high affinity muscimol binding is δ subunit dependent. Association of [³H]muscimol binding to forebrain (n=8 independent experiments in both mouse lines using individual forebrains in each experiment) and cerebellar (n=3 independent experiments using pools of 3 individual cerebella from the mouse line in each pool) membranes of WT and δKO mice (mean ± SEM). The experiments were performed in triplicate technical replicates. Forebrain and cerebellar membranes were incubated with 5 nM [³H]muscimol alone and in the presence of 100 µM GABA to determine non-specific binding. The incubations were terminated at various time points by filtration onto GF/B filters. The values are expressed as fmol/mg protein (left panels) and as % of binding at 15 min (right panels). Binding to δ-GABA_ARs (WT-δKO) was calculated by subtracting binding to non-δ-GABA_ARS in δKO mice from binding to WT membranes.