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Characterization of the collapsin response-mediator protein-2 interaction with calmodulin by surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC).
| SPR | |
|---|---|
| Kd (μM) | 75 ± 0.05 |
| ITC | |
| n | 0.81 ± 0.01 |
| Ka (M−1) | 5.8 × 104 ± 2.9 × 103 |
| Kd (μM) | 17 ± 0.9 |
| ΔH (kcal/mol) | −2.8 ± 0.05 |
| −TΔS (kcal/mol) | −3.8 |
| ΔG (kcal/mol) | −6.6 |
