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. Author manuscript; available in PMC: 2019 Apr 4.
Published in final edited form as: Methods Protoc. 2019 Feb 15;2(1):19. doi: 10.3390/mps2010019

Figure 10.

Figure 10.

Longitudinal Cuff Assembly; (a) Lateral silk knotting to expose the inside of the micro- polyurethane tube. (b) Piercing of the exterior surface of the tube (0.5 mm above Mark A) using a needle. The needle will eventually make a U-turn and pierce the interior surface of the tube 0.5 mm below mark B. (c) Longitudinal PI wire leads running along the length of the micro-polyurethane tube. (d) Completed longitudinal cuff. Longitudinal leads along the length of the micro-polyurethane tube are pressed against the inside wall of the tube by silk strands and secured with UV glue.