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. Author manuscript; available in PMC: 2019 Jul 10.
Published in final edited form as: Am J Reprod Immunol. 2019 Apr 11;81(6):e13111. doi: 10.1111/aji.13111

Figure 5: Effect of IL-1β on the transmission of HIV-1 across cervical mucosa.

Figure 5:

(a) IL8 (200 ng/ml) and CXCL10 (1000 pg/ml) at concentrations present in the 24 hour NGIS increased the transmission of HIV-1 across cervical mucosa in the organ culture. (b) Inhibitory drugs erythromycin (5ug/ml) against IL-8 and catechin-hydrate (20ng/ml) against CXCL10 decreased the transmission of HIV-1 across the mucosa. (c) IL-1β (2500pg/ml) in concentration present in the 24 hour NGIS increases the transmission of HIV-1 across cervical mucosa in the organ culture. (d) Addition of IL-1β inhibitor (10ug/ml trans-chalcone) decreased the transmission of HIV-1. (e) Addition of the IL-1β (2500pg/ml) on the cervical tissues increased the CXCL10 and IL8 in the tissues at the mRNA level. (f) IL-1β as well as a combination of CXCL10 and IL8 increased the HIV-1 transcription in the tissues compared to media exposed control. (g) Monoclonal antibodies against IL-1β (10ug/ml) in the NGIS along with HIV-1 decreased transmission compared to NGIS and HIV-1 alone. P=<0.05 was considered as significant and either parametric t tests or T-Test Unequal Variance was used to calculate for the p values.