Table 1:
Fold changes of DEGs (Differentially expressed genes) up-regulated upon NG exposure on cervical tissues compared to tissues exposed to media control
| Sample Number | CXCL10 | CXCL3 | CCL20 | TNFAIP6 | IL8 | IL6 | IL-1β |
|---|---|---|---|---|---|---|---|
| E-9202 (NG) | 3.39 | 3.32 | 2.38 | 9.32 | 1.6 | 2.46 | 1.68 |
| E-9235 (NG) | 0.18 | 0.73 | 0.74 | 0.79 | 0.57 | 0.59 | 0.75 |
| E-9249 (NG) | 6.06 | 5.35 | 17.63 | NA | NA | NA | NA |
| E-9326 (NG) | 5.82 | 61.82 | 113.77 | 44.63 | 174.85 | 106.15 | 30.91 |
| E-9395 (NG) | 7.26 | 5.82 | 26.72 | 8.63 | 8.51 | 5.24 | 4.29 |
| E-9431 (NG) | 19.56 | 4 | 2.79 | 14.12 | 4.66 | 4.29 | 2.89 |
| E-9318 (NG) | 26.17 | 3.41 | 4.89 | 7.46 | 7.67 | 4.32 | 8.4 |
| E-9457 (NG) | 13.83 | 5.94 | 10.41 | 3.07 | 42.22 | 3.48 | 8.17 |
| E-9727 (NG) | 4.29 | 15.78 | 6.59 | 2.91 | 6.06 | 10.85 | 22.94 |
| E-9761 (NG) | 3.81 | 3.12 | 34.54 | 3.39 | 15.24 | 5.13 | 6.96 |
| Average Fold Change (n=10) | 9.04 * | 10.93 * | 22.05 * | 10.48 * | 29.04 * | 15.84 * | 9.67 * |
| p value | 0.0041 | 0.0039 | 0.0039 | 0.0078 | 0.0117 | 0.0078 | 0.0078 |
The upregulated genes found in the transcriptome analysis was verified using RT-PCR on additional tissues (n=10). Numbers indicate fold changes of DEGs upon NG exposure on cervical tissues compared to tissues exposed to media control.
*
All the genes confirmed to be upregulated were statistically significant. A non parametric paired t test was used for calculating the statistical significance.