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. Author manuscript; available in PMC: 2020 Mar 2.
Published in final edited form as: FASEB Bioadv. 2019 Apr 12;1(7):404–414. doi: 10.1096/fba.2018-00075

Figure 2. Polysome profiling analysis from MM cell lines.

Figure 2.

Cytosolic extracts from cells were loaded onto a 7%−47% Sucrose gradient and fractionated with recording constant UV (254nm) monitoring. 40S, 60S, 80S and polysomes represent small and large ribosomal subunits, monosomes and multiple ribosomes per mRNA. (A) MM1.S cells overexpressing ACA11 or control empty vector (pLKO.1). (B) ACA11 knockdown in ACA11-overexpressing H929 cells.