Skip to main content
. Author manuscript; available in PMC: 2019 Nov 18.
Published in final edited form as: Stem Cells. 2019 May 3;37(7):937–947. doi: 10.1002/stem.3015

Fig 2. p53-dependent suppression of glycolysis in FA HSCs.

Fig 2.

(A) Increased p53 and TIGAR proteins in FA HSPCs. Whole cell lysates (WCL) were extracted from 30,000 LSK isolated from WT, Fanca−/−, p53−/− or p53−/−Fanca−/− mice followed by immunoblotting using antibodies against p53, TIGAR, or β-actin. (B) Deletion of p53 increases F2,6BP level in Fanca−/− HSCs. SLAM cells isolated from WT, Fanca−/−, p53−/− or p53−/−Fanca−/− mice were subjected to MicroAssay for F26BP. (C, D) Deletion of p53 increases glucose consumption and lactate production in FA HSCs. SLAM cells isolated from WT, Fanca−/−, p53−/− or p53−/−Fanca−/− mice were subjected to glucose uptake (C) and lactate production analysis (D). Results depicted in B-D are means ± SD of three independent experiments. (n=6-9 per group). *, p<0.05; **, p<0.01.