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. Author manuscript; available in PMC: 2019 Nov 20.
Published in final edited form as: Ann N Y Acad Sci. 2019 Jun 11;1454(1):3–13. doi: 10.1111/nyas.14119

Figure 1.

Figure 1.

Overview of experimental design, dissection technique, and whole body tissue clearing. A: Flow diagram of experimental design. B, C: Ventral views of eviscerated, perfused TH:Tomato mouse (B), Dbh:Tomato mouse (C) using stereomicroscope. D: Ventral view of eviscerated, perfused wild-type mouse 96 h post-PRV-GFP injection into the right iBAT pad using stereomicroscope. Note labeling of right, but not left sympathetic chain ganglia. E: Confocal microscope image of the whole upper body of a mouse after iDISCO immunohistochemistry with TH and tissue clearing. Note labeling of the bilateral sympathetic chain, neck nerves, and brain areas. TH, tyrosine hydroxylase; DBH, dopamine beta-hydroxylase; PRV, pseudorabies virus; GFP, green fluorescent protein; SG, stellate ganglion; T1–T7, ganglia for thoracic levels 1–7.