(A) Representative image of wild-type (wt) periovarian WAT (periWAT). (B) Representative image of Abcc5−/− (ko) periovarian WAT (periWAT). (C) Representative image of wt interscapular BAT (iBAT). (D) Representative image of Abcc5−/− interscapular BAT (iBAT). (E) Total fat mass. Each data point represents mean±SEM. Changes in total fat mass over time for Abcc5−/− (ko) vs wt were analysed by two-way ANOVA with a Bonferroni post-hoc test, ***P≤0.001. The insert shows Western blot analysis of ABCC5 protein expression in adipose tissue. Lanes 1 and 2, periovarian WAT (periWAT) from female Abcc5−/− mice and female wt mice respectively; lanes 4 and 5, intrascapular BAT (iBAT) from female Abcc5−/− and female wt mice respectively. (F) Mass of periWAT and (G) mass of iBAT, wt n=8, Abcc5−/− n=6 animals. (H) Lipid plasma levels for female Abcc5−/− mice (n=14) and female wt mice (n=15). Data shown as mean±SEM; Welch’s unequal variances t-test, *P<0.05, ***P<0.001.