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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Cancer Immunol Immunother. 2018 Apr 25;67(7):1091–1103. doi: 10.1007/s00262-018-2164-6

Fig. 4. Poly-ICLC stimulates MDA5 more effectively than poly-IC through the proton sponge effect.

Fig. 4

a–b, The CD8aDC cell line was stimulated with various doses of poly-IC or poly-ICLC and 18 h later the levels of IFN-I and IL12p40 were measured in the supernatants. c–d, BM DCs from WT, TLR3-KO, MDA5-KO or TLR3/MDA5 KO mice were stimulated with 10 μg poly-IC or poly-ICLC and 18 h later the levels of IFN-I and IL12p40 were measured in the supernatants. e–f, WT mice were injected (i.v.) with 50 μg poly-ICLC or poly-IC and 18 h later the amounts of IFN-I and IL12p40 were measured in serum. g–h, WT, TLR3-KO, MDA5-KO or TLR3/MDA5-KO were injected (i.v.) with 50 μg poly-ICLC and 18 h later the amounts of IFN-I and IL12p40 were measured in serum. i, The CD8aDC cell line was incubated with bafilomycin A1 6 h before stimulation with 50 μg/ml poly-ICLC and the production of IFN-I and IL12p40 was measured. Results are presented for individual replicate samples/mice (each symbol) with the mean ± SD for each group.