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. Author manuscript; available in PMC: 2019 Oct 7.
Published in final edited form as: Mol Microbiol. 2019 Jun 12;112(3):800–819. doi: 10.1111/mmi.14317

Fig. 6.

Fig. 6.

Scl1-mediated GAS attachment to ECM-deposited by cancer-associated fibroblasts (CAFs). Isogenic WT M1 and M41 GAS strains, their Δscl1 mutants, and trans-complemented strains to restore Scl1 expression in each mutant, or express Scl.chimera2, were compared for the attachment to CAF-derived ECM. GFP-GAS strains were inoculated onto CAF-derived ECM coatings, allowed to attach for 1 h, and imaged using fluorescent confocal microscope with 100x objective. Top, representative images of attached strains are shown. Bottom, quantification of GAS attachment. Bacteria were counted in 20 random fields, and the average from all 20 fields was calculated with WT binding set as a 100%. Statistical significance was calculated using a one-way ANOVA from three independent experiments, each performed in duplicate wells (N=3±SD); **P≤0.01, ***P≤0.001. Statistical analysis evaluates the difference between adherence to CAF-derived matrices by the WT and their respective isogenic Δscl1 mutants. Each symbol shown represents one imaged-field.