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. Author manuscript; available in PMC: 2009 Sep 18.
Published in final edited form as: J Neurosci. 2009 Mar 18;29(11):3463–3474. doi: 10.1523/JNEUROSCI.5655-08.2009

Figure 1. GFP expression in retinal ganglion cells after in utero retinal electroporation.

Figure 1

(A) Cartoon depicting in utero retinal electroporation procedure. All images are taken from E18.5 embryos electroporated with GFP at E14.5. (B–C) GFP+ retinal cells are clearly visible in both whole mount (B) and cryosectioned retina (C), where the GFP+ cells are predominantly localized to the ganglion cell layer. (D–E) GFP+ axons can be visualized throughout the optic nerve (ON), optic chiasm (OC) and optic tract (OT) in either the semi-intact visual system preparation (D) or serial cryosections throughout the projection pathway (E). NF = neurofilament, Vit = vitreous. All scale bars = 100 µm.