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. Author manuscript; available in PMC: 2009 Sep 18.
Published in final edited form as: J Neurosci. 2009 Mar 18;29(11):3463–3474. doi: 10.1523/JNEUROSCI.5655-08.2009

Figure 3. Ectopic EphB1 and Zic2 induce an increase in GFP+ fibers in the ipsilateral optic tract.

Figure 3

It is possible that the apparent increase in ipsilateral projections actually arises from a decrease in contralateral projections. To confirm that this is not the case, we divided the GFP+ signal in the ipsilateral optic tract by the GFP+ area in the retina. If there is not an actual increase in uncrossed fibers, then this ratio should remain constant for all conditions. However, it is clear that there are significantly more GFP+ axons in the ipsilateral optic tract of EphB1 and Zic2-electroporated embryos. n ≥ 10 embryos for each condition, from ≥ 3 electroporation experiments. Data represent mean ± s.e.m., all scale bars = 100 µm. ANOVA: F(3,68) = 112.10, p < .0001. Modified t-tests: * = p ≤ .05, *** = p < .0001.