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. Author manuscript; available in PMC: 2009 Sep 18.
Published in final edited form as: J Neurosci. 2009 Mar 18;29(11):3463–3474. doi: 10.1523/JNEUROSCI.5655-08.2009

Figure 6. Ex vivo retinal electroporations reveal that EphB1 and EphB2 are expressed at different surface levels.

Figure 6

(A) Cartoon diagramming ex vivo retinal electroporation. DNA is injected into dorsal retina and electroporated. Retinae are removed and cultured in serum-free medium (SFM) for ~40 hours, and then explants are prepared from GFP+ retinal regions and plated on laminin (for live HA staining) or adjacent to ephrin-B2 substrate borders. (B) Live HA staining reveals that EphB2 is expressed at higher levels compared to RGC axons from EphB1, KD EphB1 and 2Y-E EphB1 electroporated retina. Scale bar = 20 µm.