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. Author manuscript; available in PMC: 2019 Jul 12.
Published in final edited form as: J Mol Biol. 2019 May 31;431(15):2777–2789. doi: 10.1016/j.jmb.2019.05.035

Table 1.

3H-TPP+ binding to EmrE mutants in DDM.1

EmrE mutant Kd (nM)
Wild type 0.8 ± 0.1
M21G 4.0 ± 0.7
A59L 23.4 ± 2.4
S64V 1.0 ± 0.6
G90V 5.3 ± 0.6
I101G 2.6 ± 1.3
N102A 2.3 ± 0.4
N102V 1.2 ± 0.2
1

Assays performed on ice, at pH 8. Binding affinity of TPP+ is in nM range due to higher pH and lower temperature [5,36]