. Author manuscript; available in PMC: 2019 Jul 12.

Published in final edited form as: J Mol Biol. 2019 May 31;431(15):2777–2789. doi: 10.1016/j.jmb.2019.05.035

Table 2.

S64V- and WT-EmrE drug binding affinity by ITC¹

Substrate	K_d (μM)	ΔH (kJ/mol)	n
S64V (DMPC/DHPC bicelles)
TPP⁺	0.6 ± 0.1	−52 ± 2	0.50 ± 0.03
EtTPP⁺	11.4 ± 0.1	−51 ± 5	0.51 ± 0.04
MeTPP⁺	60 ± 10	−40 ± 10	0.49 ± 0.01
WT (DMPC/DHPC bicelles)
TPP+	0.5 ± 0.1	−29 ± 4	0.49 ± 0.03
WT (DLPC/DHPC bicelles) [32]
TPP⁺	0.45 ± 0.01	−22 ± 1	0.49 ± 0.05
EtTPP⁺	21.8 ± 0.7	−16.6 ± 0.2	0.57 ± 0.04
MeTPP⁺	130 ± 20	−12.7 ± 0.2	0.60 ± 0.04

Assays performed at 45°C, pH 7.