Skip to main content
. Author manuscript; available in PMC: 2020 Feb 28.
Published in final edited form as: Curr Genet. 2019 Jun 22;66(1):51–58. doi: 10.1007/s00294-019-01006-5

Fig. 3.

Fig. 3

Apj1 domain analysis for Hsp104-mediated elimination of strong [PS1+] variants, a Strong [PSI+]STR and [PSI+]Sc4 cells of the W303 genetic background lacking genomic APJ1 were transformed with plasmids expressing wild-type Apj1. Apj1-J-RHA, the first 161 residues of Apj1, or the Y/A chimera of Ydj1 and Apj1. Cells were then transformed with a plasmid overexpressing Hsp104 (GPD-HSP104) that normally cures [PSI+] Color phenotype assays are shown for representative transformants (n = 10 for each variant), b Lysates of representative cells from panel A were resolved by SDD-AGE and subjected to immunoblot analysis using polyclonal antibody raised against Sup35. c Lysates of representative cells from panel A were resolved by SDS-PAGE and subjected to immunoblot analysis using polyclonal antibody raised against Apj1. Load control shown is a nonspecific protein cross-reacting with our Apj1 primary antibody