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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: ACS Pharmacol Transl Sci. 2019 Mar 18;2(2):92–100. doi: 10.1021/acsptsci.8b00048

Figure 3.

Figure 3.

Pirin interacts with the MRTF/SRF pathway. (A) CCT251236 inhibits Q231L Gα12 activated SRE.L luciferase in HEK293T cells; n = 2 (B) Overexpression of C-terminally V5 tagged pirin induces SRE.L luciferase signal in HEK293T cells. Luciferase signals were normalized to cell viability and the pcDNA control, and results are expressed as the mean ± SEM. Individual means are represented by filled circles. *, p < 0.05 using an unpaired t test; n = 3 (C) Knockdown of pirin also reduces MRTF-A driven SRE.L luciferase in primary dermal fibroblasts after overexpression of MRTF- A. Luciferase signals were normalized to cell viability and the pcDNA siControl, and results are expressed as the overall mean as well as individual paired mean values. n = 3. (D) Validation of pirin knockdown using qPCR. Results are expressed as the mean ± SEM as well as individual mean values ***, p < 0.001 using an unpaired t test; n = 3.