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. Author manuscript; available in PMC: 2009 Sep 16.
Published in final edited form as: Br J Cancer. 2008 Sep 16;99(6):949–956. doi: 10.1038/sj.bjc.6604632

Figure 2.

Figure 2

Western blot analysis of MUC4 expression in gastric cancer cell lines. Total protein lysates from AGS, KATOIII and MKN45 gastric cancer cells were prepared. Protein lysate from FG (pancreatic cancer cell line) cells was taken as a positive control. Protein lysates were electrophoretically resolved on 2% agarose gel. Resolved proteins were transferred onto PVDF membrane and probed with MUC4 MAb (8G7) and detected using Amersham HRP-conjugated secondary antibody and ECL kit. Immunoblot of β-actin, obtained from 10% SDS–PAGE/Western, was used as an internal control to correct for the loading variation.