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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Development. 2009 May;136(9):1443–1452. doi: 10.1242/dev.029447

Table 1. Primers (5′ to 3′) for real-time PCR.

Human gene Forward Reverse
GAPDH TCGACAGTCAGCCGCATCTTCTTT ACCAAATCCGTTGACTCCGACCTT
GLI1 GGCTGCAGTAAAGCCTTCAGCAAT TGCAGCCAGGGAGCTTACATACAT
GLI2 AGGACAAGGCCAACATCGTCATCT TCCCTGGCTTTCATTTGCAGTTGG
GLI3 TTGCACAAAGGCCTACTCGAGACT CTTGTTGCAACCTTCGTGCTCACA
NKX2.2 GACAACTGGTGGCAGATTTCGCTT AGCCACAAAGAAAGGAGTTGGACC
OLIG2 GGTAAGTGCGCAATGCTAAGCTGT TACAAAGCCCAGTTTGCAACGCAG
SHH AAGATCTCCAGAAACTCCGAGCGA CGAGATGGCCAAAGCGTTCAACTT