Table 2. Antibodies.
Antibody | Isotype | Dilution | Source | Cat. # |
---|---|---|---|---|
OLIG2 | Goat IgG | 1:500 | Santa Cruz | sc-19969 |
NKX2.2 | Mouse IgG | 1:50 | DSHB, Iowa City, IA | 74.5A5 |
βIII-tubulin | Mouse IgG | 1:5000 | Sigma | T 8660 |
GFAP | Rabbit IgG | 1:5000 | DAKO | Z0344 |
NG2 | Mouse IgG | 1:400 | BD Pharmingen | 554275 |
HB9 | Mouse IgG | 1:50 | DSHB, Iowa City, IA | 81.5C10 |
PDGFRα | Rabbit IgG | 1:1000 | Gift from Dr William Stallcup | |
O4 | Mouse IgM | 1:50 | Chemicon | MAB345 |
MBP | Rabbit IgG | 1:500 | Chemicon | AB5622 |
Human nuclei | Mouse IgG | 1:200 | Chemicon | MAB1281 |