Figure 4.

CG neurons ex press α₆, α₃, and β₁, but not α₂ or α₇, in situ. Cryostat sections of E7-8 ciliary ganglia were stained with anti-integrin antibodies as described in Materials and Methods. A and B show a double-labeled section incubated with anti-β₁, mAb (TASC, A) and a polyclonal antibody to neurofilament (B). Anti-β₁ immunoreactivity appears to be highly coincident with that of the neuronal marker, notably in structures which appear to be axons (arrows). Other sections were incubated with antibodies to α₃ (cytoplasmic domain A-form, C), α₆ (D), α₆ preimmune IgG (E), α₂ (cytoplasmic domain, F), and α₇, mAb (G). As for β₁, α₃ and α₆ immunoreactivity often colocalizes with that of neuronal markers (data not shown). The α₂ and α₇ antibodies were immunoreactive with control tissues (epithelium and muscle, respectively) in the same experiments (data not shown). Scale bar, 100 μm.