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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Cell Sci. 2009 Jun 1;122(Pt 11):1842–1851. doi: 10.1242/jcs.043257

Figure 4. Size-Reduced Olfactory Bulbs in Adult β8-/- Mice.

Figure 4

(A, B); Gross images of P90 brains from wild type (A) and β8-/- (B) littermates that were sacrificed and cardiac-perfused with Bouin's fixative. In comparison to the wild type mouse (arrow in A), note the obvious size-reduced olfactory bulbs in the β8-/- animal (arrow in B). (C); Microscopic images of H&E-stained coronal sections through olfactory bulbs of P90 wild type and β8-/- mice. Note that β8-/- olfactory bulbs are significantly smaller, but have an apparently normal olfactory bulb cytoarchitecture. Abbreviations: GLO, glomerular layer; EPL, external plexiform layer; MCL, mitral cell layer; IPL, internal plexiform layer; GCL, granule cell layer; RMS, rostral migratory stream. (D, E); Coronal sections through olfactory bulbs taken from P90 wild type (D) and β8-/- (E) mice were immunofluorescently labeled with anti-DCX (green) to visualize neuroblasts and differentiated neurons and anti-GFAP (red) to identify astrocytes. Note that fewer DCX+ neuroblasts (green) are present in the RMS of β8-/- olfactory bulb versus the wild type control (arrowheads in D, E). However, similar numbers of GFAP+ astrocytes (red) and differentiated neurons (green) are present in the olfactory bulbs of β8-/- mice in comparison to wild type control mice (arrows in D, E). Abbreviations: RMS, rostral migratory stream; DCX, doublecortin; GFAP, glial fibrillary acidic protein.