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. Author manuscript; available in PMC: 2009 May 26.
Published in final edited form as: J Neurosci. 2009 Jan 28;29(4):1224–1234. doi: 10.1523/JNEUROSCI.4858-08.2009

Figure 6. Immunoprecipitation experiments show D2R-DAT interaction in vivo.

Figure 6

Striatal membranes were prepared from WT and D2R−/− mice. 500 μg proteins were used for Immunoprecipitation experiments using either DAT or D2R specific antibodies. A) Samples were immunoprecipitated with anti-DAT and reveled with anti-D2R antibodies (first two lanes). Note the presence of immunoprecipitated D2R only in WT striata but not from D2R−/− striatal membranes; C-, control experiments were performed using normal IgG rabbit. Western Blots (WB) were performed using 50 μg striatal membranes showing the presence of D2R only in WT extracts. B) Same as in A, but proteins were precipitated with anti-D2R antibody and revealed with anti-DAT antibody; C-, control experiments were performed using normal IgG mouse. Note that DAT was precipitated only from WT extracts. Western Blots as in A were revealed using anti-DAT antibody showing presence of DAT in WT and D2R−/− extracts. Actin was used as internal control of loaded quantities of striatal extracts in A and B.