Skip to main content
. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: J Neurosci. 2009 May 20;29(20):6568–6579. doi: 10.1523/JNEUROSCI.0181-09.2009

Figure 3. Ca2+ entry and intracellular Ca2+ facilitate somatodendritic DA release.

Figure 3

a. Average [DA]o versus time profiles evoked by local stimulation (30 pulses, 10 Hz) in the SNc in the absence and presence of a non-selective Ca2+ channel blocker, cadmium (Cd2+, 100 μM, n = 6). b. Average [DA]o versus time profiles in SNc in the absence and presence of a fast acting Ca2+ chelator BAPTA-AM (BAPTA, 50 μM, n = 6). c. Summary of the effect of Cd2+ and BAPTA on peak [DA]o; evoked [DA]o was measured at the time point of control peak [DA]o, which was taken as 100%. Blockade of stimulus-induced Ca2+ entry by Cd2+ abolished evoked [DA]o (n = 6, ***p < 0.001 vs. control) confirming that Ca2+ entry is required to trigger evoked somatodendritic DA release. Buffering of stimulus-induced intracellular Ca2+ by BAPTA decreased evoked [DA]o (n = 6, **p < 0.01 vs. control), demonstrating the involvement of intracellular Ca2+ in evoked somatodendritic DA release.