A. Responses to 3 second applications of LY341495 and capsaicin, first separately and then together. Also shown in the right panel is the predicted response to simultaneous application based on linear summation. B. Similar to (A) except that the duration of the application was 1 second. Note that for subsaturating application of drugs, the predicted response more closely approximates the response to simultaneous application of LY341495 and capsaicin. Same cell as in (A). C. Comparison of evoked and predicted responses to simultaneous application of LY31495 and capsaicin as a function of puff duration. Long applications were for a duration of 3 seconds, while brief applications were either 1 second or 0.5 seconds. Charge was obtained by integration of current during the period of drug application. For both the peak response and the total charge transfer, there was no significant difference between the size of the predicted and actual response to short puffs (p>0.5, n=7), but the response to long puffs was significantly smaller than predicted based on summation (p<0.01, n=7).