Effect of P529, RT, and P529 + RT and the combination on p-Akt, total Akt, Bcl-2, and Bax. Ionising radiation causes a > 10-fold increase in p-Akt levels. However, when P529 is added to the treatment, p-Akt levels are lower than those observed in untreated cells. Total Akt levels are also increased in RT-treated cells, but levels return to normal when the drug is added. Bcl-2 decreases as a consequence of P529, RT, and combination treatment, whereas Bax increases in RT- and RT + P529-treated cells. The densitometric analysis shows that the Bcl-2/Bax ratio is reduced in RT and RT + P529 cells. The Y-axis represents fold changes with respect to control.