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SZP immunohistochemistry of bilayered and Knee constructs. SZP immunohistochemistry found the presence of the protein on the surfaces of the SZC layer of bilayered constructs. Stronger staining was found in the 3S2M construct compared to the 2S2M group. No staining for SZP was found for the MDZC layer in either bilayered group (only 3S2M shown). Dispersed SZP was found in Knee constructs with a mixed-population of chondrocytes. Scale bar = 100 µm. Cell nuclei are counterstained.
