Figure 2. Genetic deletion of PSD-95 does not affect mRNA levels of 5- HT_2A and 5-HT_2C receptors or mRNA editing of the 5-HT_2C receptor.

(A) Cortical 5-HT_2A receptor mRNA levels (N=4 littermate pairs; 11 measurements were performed for each animal) and hippocampal 5-HT_2C receptor mRNA levels (N=4 littermate pairs; 5 measurements for each animal) normalized to β-actin mRNA levels as measured by quantitative RT-PCR. There are no changes in 5-HT_2A mRNA levels and no changes in 5-HT_2C mRNA levels in the absence of PSD-95. (B) 5-HT_2C mRNA editing frequencies at 5 edited sites. The frequency of editing at the 5 sites is not significantly different in PSD-95^null mice. (C) and (D) Frequencies of the different edited isoforms detected (wildtypes, N=94; nulls N=93). 15 isoforms were detected, and 14 of them were not significantly altered in the absence of PSD-95. There is a significant increase in the proportion of the VSI isoform in PSD-95^null mouse hippocampus. All mRNA editing data are plotted as the frequency expressed as a fraction of the total, +/-the SEM. Normalized mRNA measurements are presented as means +/− SEM. ^*p < .05, ^**p < .01, ^***p < .001; one-tailed unpaired t-test for the comparison of mRNA levels and one-way ANOVA followed by Newman-Keuls post-hoc tests for comparison of the mRNA editing measurements.