Fig. 1.

Effect of pimecrolimus on eosinophil superoxide production. Mean superoxide anion (${\text{sO}}_2^{-}$) production by purified eosinophils from normal (n = 13) or atopic donors (n = 9 or 10) is shown following stimulation with PAF, IL5 or immobilized sigA after pre-incubating eosinophils with no pimecrolimus (i.e. 0 nm pim; solid line) or with pimecrolimus at 1 nm (long dashes), 10 nm (medium dashes), 100 nm (short dashes) or 1,000 nm (dotted). Positive (PMA, closed circles) and negative (medium alone, open circles) control responses are shown (n = 11 for normals and n = 4 for atopics). Significant changes in superoxide anion production relative to stimuli alone (i.e. 0 nm pimecrolimus) at the 180-min time point are indicated by asterisks. * p < 0.05; ** p < 0.01.