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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Gen Virol. 2009 May 27;90(Pt 9):2191–2200. doi: 10.1099/vir.0.012104-0

Figure 5. Organisation of DAV genome.

Figure 5

A. The 4806 nt DAV genome encodes two major ORFs and three additional small ORFs that contain over 50 amino acids. The reading frames as shown start with an AUG codon and the extension on the capsid ORF indicates this reading frame overlaps with the RdRP reading frame but the first methionine is downstream of the RdRP reading frame B. Analysis of the 5' proximal ORF showed high similarity of DAV replicase regions with members of the Tetraviridae and Birnaviridae families. These viruses have a permuted RdRp, with the GDD region upstream of other palm sub-domains. DAV replicase also showed this permuted arrangement, as indicated by the C-A-B. C. Mass spectrometry analysis indicates that the 3' proximal large ORF encodes the capsid protein. The deduced sequence of the capsid protein is shown and the peptides identified by MALDITOF (bold) and LC-MS-MS (underlined) indicated.