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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Neurosci. 2009 Jul 1;29(26):8321–8328. doi: 10.1523/JNEUROSCI.6191-08.2009

Figure 6. Functional effects of the substitution of native residues by cysteines and their endogenous disulfide crosslinking.

Figure 6

Eight pairs of Cys-substituted α and Cys-substituted β4 were expressed in HEK 293 cells. Recordings were in outside-out macropatches with 10 µM Ca2+ in the pipette. Black bars show the effects due to the disulfide, and gray bars show the effects due to the substitution of the two Cys for native residues. Effects on V50 (A), the rate constant for opening (B), and the rate constant for closing (C) were extrapolated to 100% crosslinking or 100% reduction as previously described (G. Liu et al., 2008b).