Fig. 3.

Upregulation by IFN-γ of β₂m, LMP2, LMP7, TAP1 and TAP2 transcripts in the RCC cell lines a RCC52, b RCC98, c HH050, d HH244, e HH332, f HOKN-9 and in the control g NP69 cell line. Total RNA from cells cultured with or without IFN-γ (300 U/ml) at 37°C for 48 h was isolated and transcribed. β₂m, LMP2, LMP7, TAP1 and TAP2 transcripts were amplified by PCR with appropriate primer pairs. GAPDH was included as an internal control. The PCR products were run on 1% agarose gel and stained with ethidium bromide. The upper frame is banding patterns in agarose gel and the lower one is the result of quantification. GAPDH was also included as an internal loading control. The PCR products were analyzed by agarose gel electrophoresis. Experiments were repeated three times with each cell line; similar results were obtained