Fig. 5.

Monolayer culture morphology and sequence abnormalities of the β₂m genes of the RCC52 cell line and its representative clonal sublines. a RCC52, parental cells showing predominantly epithelioid cell morphology; clonal sublines E2G5 and E4F5 displaying epithelioid cell morphology; and clonal subline M3B8 showing fibroblast-like cell morphology. Sequence chromatograms of the β₂m gene show the double mutations, delG and delCT in the clonal sublines M2C5 and M3B8 with fibroblast-like morphology b and the single mutation in the clonal sublines E3D2, E1A5, E2G5, E3D4, E4D7 and E4F5 with epithelioid cell morphology. c The representative sequence chromatogram of the clonal subline E4F5 is shown, The sequence chromatogram of RCC98 cells which contains quadruplicate CT dinucleotide is shown for comparison. Original magnification, ×100