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. Author manuscript; available in PMC: 2010 Jan 8.
Published in final edited form as: J Neurosci. 2009 Jul 8;29(27):8743–8751. doi: 10.1523/JNEUROSCI.2294-09.2009

Figure 6. Purification of phosphorylated PrP from PrP/Cdk5/p25 co-transfected N2a cells protein extracts.

Figure 6

A-D. Western blot analyses with 3F4, phospho-tyrosine (pTyr), anti-pPrPS43 and β-actin on phospho-column fractionated proteins from PrP/Cdk5/p25 co-transfected N2a cells (A), roscovitine-treated PrP/Cdk5/p25 co-transfected N2a cells (B), PrP S43A/Cdk5/p25 co-transfected N2a cells (C), PrP-transfected N2a (D), or untransfected N2a cells (E).