Table I. Conservation of phosphorylation motifs in PrP amongst various species.

Mammalian species examined are human, guar, macaque, kudu, bison, rhesus monkey, baboon, mink, rabbit, camel, rat, nilgai, sheep, mouse, hamster, cattle and cat. Human Doppel was also included. The phosphorylation sites were identified with NetPhos 2.0 Server in EXPASY and had significant scores except the last SP motif(*), which has low predictability for phosphorylation.

Putative motif	Phospho AA	Mammals	Chicken	Turtle	Doppel
PGQSPGGN	S43	All	RQ	SN	-
IHFGSDYED	S143	Only in human, bovine and bison	S	R	A
FGSDYEDRY	Y145	W in rat, mouse and hamster	D	E	-
YEDRYYREN	Y149	All	WW	WW	YY
PMDEYSNQN	Y169	All	-	R	A
HTVTTTTKG	T191	All	P	P	F
TVTTTTKGE	T192	All	A	N	Q
VTTTTKGEN	T193	All	A	E	K
YQRGSSMVL	S230	All except rabbit	S	S	A
QRGSSMVLF	S231	All except rabbit	G	G	G
VLFSSPPVI*	S237	All	AD	DP	HQ